In Vitro Antibody Production of Human Tonsil for Sheep Red Cells (SRC)

Abstract

An attempt to determine the capacity of lymphocytic suspension from human tonsils to produce antibodies in vitro against sheep red cell was conducted using Marbrook's culture technique. Detection of the antibodies was made using Cunningham's plaque-forming method. It was confirmed that the plaque-forming cells increased predominantly on the 6th day of culture. There was no relationship between the age of the patient and the plaque-forming capacity. The immunological roles of the human tonsil, with additional reference to E- and EAC-binding lymphocytes previously reported, become more pronounced.