Multiplex analyses of cytokine and chemokine release from the cultured fibroblast of nasal polyps: the effect of IL-17A

Abstract

Conclusion: Our results demonstrate for the first time a potentially enhanced basal secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin (IL)-17A-stimulated secretion of IL-6 from nasal polyp fibroblasts, enhanced basal secretion of IL-6 from eosinophilic nasal polyp fibroblasts, and a remarkable up-regulation of IL-9 and granulocyte colony-stimulating factor (G-CSF) from nasal fibroblasts by IL-17A stimulation. Objectives: The fibroblast, one of the main cell types making up nasal polyps, is thought to be a target cell of various cytokines. Methods: Subcultured fibroblasts were established from human polyp biopsy tissues. Simultaneous quantification of 27 kinds of cytokines and chemokines in culture supernatants in unstimulated and IL-17A-stimulated conditions was performed with a human multiplex cytokine assay system. Results: The IL-17A receptor was expressed at similar levels in all three groups. In the eosinophilic group, basal secretion levels of IL-6 were significantly higher than those in the control and non-eosinophilic groups. Basal secretion of MCP-1 in both the non-eosinophilic and eosinophilic groups was also higher than that of the control group. Both IL-9 and G-CSF secretion were remarkably enhanced by IL-17A stimulation in all three groups. The receptor-mediated response by IL-17A significantly up-regulated IL-6 release alone in the non-eosinophilic and eosinophilic groups as compared with the control group.

Keywords::

• Chronic rhinosinusitis
• IL-6
• MCP-1
• IL-9
• G-CSF
• basic FGF

Acknowledgments

We thank Takako Ikegami and Tomomi Ikeda, Laboratory of Molecular and Biochemical Research, Research Support Center, Juntendo University Graduate School of Medicine, Tokyo, Japan, and Akemi Koyanagi, Division of Cell Biology, Biomedical Research Center, Juntendo University School of Medicine for technical assistance. This study was supported by a research grant from the Ministry of Education, Science, and Culture of Japan (nos. 22791631, 22791632, and 22791633).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.