Identification of novel targets for seasonal allergic rhinitis during and outside the pollen season by microarray analysis

Abstract

Conclusion: FOS, JUN, and DUSP1 could be used as candidate targets for the treatment of seasonal allergic rhinitis (SAR) during the pollen season, while KLF4 and CD163 could be used as candidate targets outside the pollen season. Objectives: The aim of this study is to screen novel genes related to SAR during and outside the pollen season, by using microarray analysis. Methods: The mRNA expression profile (GSE50101) of CD4⁺ T cells from SAR and healthy controls during and outside the pollen season was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) were then screened with the cut-off criteria of fold change larger than 1.5 and p-value less than 0.05. Results: A total of 47 DEGs were identified. Ten DEGs were shared by SAR patient samples during and outside the pollen season, while four and 23 DEGs were specific to during and outside the pollen season, respectively. Five miRNAs were screened in this study. Among these miRNAs, miR-139, miR-101, miR-29A, and miR-181 could target FOS; miR-200 and miR-29A could target KLF4; miR-101 and miR-200 could target DUSP1; miR-139 and miR-181 could target JUN and CD163, respectively.

Keywords:

• Seasonal allergic rhinitis
• differentially expressed genes
• microRNA
• network analysis

Acknowledgments

This work was supported by grants from Jiangsu Provincial Administration of traditional Chinese medicine Program (LZ13115); Supported by Nanjing medical Science and technique Development Foundation; Supported by Jiangsu Province ordinary university graduate students scientific research innovation projects In 2013.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.