Changes in circulating follicular helper T-cells in Chinese patients with allergic rhinitis

Abstract

Conclusions: Changes of circulating Follicular helper T (cTfh) cells existed in allergic rhinitis (AR) patients, and the severity of disease was associated with a more severe change of cTfh milieu. These results imply that cTfh cells may play a crucial role in the pathology of AR in Chinese patients. Objectives: The aim of this study was to investigate the changes in cTfh cells in Chinese AR patients.

Methods: Fifty-two patients were studied (32 in the AR group and 20 in the control group) for this research. The cTfh cell frequency and mRNA levels of transcription factor Bcl-6, B lymphocyte induced maturation protein 1 (BLIMP-1), and related cytokine IL-21 (IL-21 protein was also measured) were analyzed. Clinical severity was evaluated by total serum IgE levels, visual analog scale scores (VAS), and rhino-conjunctivitis quality-of-life questionnaires (RQLQ). Results: The frequency of cTfh cells were elevated in AR groups vs healthy controls (p < 0.05). Levels of IL-21 mRNA, Bcl-6 mRNA and the level of IL-21 protein were also significantly higher in the AR groups (p < 0.05), whereas BLIMP-1 mRNA was decreased (p < 0.05). Furthermore, positive correlations were identified between the frequency of cTfh cells and indicators of clinical severity (p < 0.01).

Keywords:

• Allergic rhinitis
• circulating Tfh cells
• clinical severity
• IgE

Acknowledgements

This study was supported by the National Natural Science Foundation of China (81271061). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.