Irradiation with narrowband-ultraviolet B suppresses phorbol ester-induced up-regulation of H₁ receptor mRNA in HeLa cells

Abstract

Conclusion These findings suggest that low dose irradiation with 310 nm NB-UVB specifically suppressed the up-regulation of H1R gene expression without inducing apoptosis and that UVB of shorter or longer wavelength than 310 nm NB-UVB had no such effects. Objective To develop a narrowband-ultraviolet B(NB-UVB) phototherapy for allergic rhinitis, this study investigated the effects of irradiation with NB-UVB at wavelength of 310 nm on phorbol-12-myristate-13-acetate (PMA)-induced up-regulation of histamine H₁ receptor (H1R) mRNA in HeLa cells. Methods The mRNA levels of H1R in HeLa cells were measured using real-time RT-PCR. Apoptosis were evaluated with DNA fragmentation assay. Results PMA induced a significant increase in H1R mRNA expression in HeLa cells. Irradiation with 305 nm UVB and 310 nm NB-UVB, but not with 315 nm UVB at doses of 200 and 300 mJ/cm² significantly suppressed PMA-induced up-regulation of H1R mRNA. At a dose of 200 mJ/cm², irradiation with 305 nm UVB, but not with 310 nm NB-UVB, induced apoptosis, although exposure of the cells to both 305 and 310 nm UVB induced apoptosis at a dose of 300 mJ/cm² after PMA treatment in HeLa cells. Conversely, irradiation with 315 nm UVB at doses of 200 and 300 mJ/cm² did not induce apoptosis.

Keywords:

• Histamine H1 receptor
• narrowband-ultraviolet B
• irradiation
• phototherapy
• allergic rhinitis

Acknowledgments

Supported in part by Grant-in-Aids from the Ministry of Health and Welfare of Japan, from the Japan Society for the Promotion of Science, and from the Osaka Medical Research Foundation for Incurable Diseases.

Declaration of interest

AF, TM, and TM are employees of Nichia Corporation. The other authors report no conflicts of interest.