Localization of histamine (H₁, H₂, H₃ and H₄) receptors in mouse inner ear

Abstract

Conclusion The present findings show that all four types of histamine receptors (H1R, H2R, H3R, and H4R) are present in the inner ear, thus supporting the hypothesis that histamine plays a physiological role in the inner ear. Objective To analyse the presence of histamine receptors in the normal mouse inner ear. Methods CBA/J mice were used in this study. The localization of H1R, H2R, H3R, and H4R in the inner ear, i.e. cochlea, vestibular end organs, vestibular ganglion, and endolymphatic sac, was studied by real-time PCR and immunohistochemistry. Results The mRNA for each receptor sub-type was detected in the inner ear. In the immunohistochemical study, the organ of Corti, spiral ganglion, vestibular ganglion, vestibular sensory epithelium, and endolymphatic sac cells showed an immunofluorescent reaction to all histamine receptors.

Keywords:

• Histamine
• histamine receptor
• inner ear
• mouse
• RT-PCR
• immunohistochemistry

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Funding statement

This study was supported by a Health and Labor Science Research Grant for Research on Specific Disease (Vestibular Disorders) from a Grant-in-Aid for Scientific Research (25462643) provided by the Ministry of Education, Science and Culture, Japan and also by the Swedish Medical Research Council (grant no. 17X-7305).