Quantitative Bacterial Culture from Adenoid Lymphatic Tissue with Special Reference to Haemophilus influenzae Age-associated Changes

Abstract

Homogenized adenoid tissue from 55 children (28–153 months) undergoing adenoidectomy because of nasopharyngeal obstruction was investigated by means of quantitative aerobic bacterial culture. The children were divided into two groups, the hypertrophy alone group—AH (n = 29)—and the hypertrophy with longstanding secretory otitis media group—SOM (n = 26). A nasopharyngeal culture was obtained preoperatively from 38 of the cases. Non-typeable H. influenzae (NTHI) was found in twice as many cases in the AH group as in the SOM group, 21/29 (72%) compared to 11/26 (42%) (p <0.05) and in a significantly higher mean concentration, 5.7 × 10⁵CFU/g compared to 1.9 × 10⁵CFU/ g(p = 0.02). For the other aerobic potentially pathogenic bacteria no such difference was found. The bulk of the NTHI-positive cases and the cases with the highest concentrations were found in the children below the age of 6 years. In the nasopharyngeal cultures NTHI alone or together with 5. pneumoniae and/or B. catarrhalis was found in 29% of the cases in both the AH group and SOM group. NTHI was found in only 50% of the nasopharyngeal cultures corresponding to a positive quantitative culture (10/20). These findings suggest that NTHI is harboured within the adenoid and could thereby chronically stimulate the local immune defense. However, the present study indicates that there is no aerobic bacterial overload in the adenoid tissue in children with SOM compared to children without middle-ear disease.

Key Words:

• non-typeable H. influenzae
• quantitative culture
• host-parasite interaction