Comparison of mesenchymal stromal cells from young healthy donors and patients with severe chronic coronary artery disease

Abstract

Background: It has been questioned whether bone marrow-derived mesenchymal stromal cells (MSCs) from patients with ischemic heart disease are suitable for use in regenerative stem cell therapy. We compared MSCs from patients with chronic coronary artery disease (CAD) and MSCs from young healthy donors with respect to phenotype, proliferation and endothelial differentiation capacity. Methods: MSCs from 16 young healthy donors and 15 elderly CAD patients were isolated, expanded by ex-vivo cultivation for two cell passages and characterized by flow cytometry, real time PCR and angiogenesis assay. Results: MSCs from healthy donors and CAD patients expressed the same surface markers and had similar proliferation capacity. In both groups VEGF-stimulation significantly increased the expression of the endothelial genes thrombospondin 1, Tie-2 and von Willebrand Factor and induced the capacity to form ring structures on extracellular matrix. Discussion: MSCs from young healthy donors and CAD patients proliferate equally well, express the same surface markers and increase in endothelial gene expression and ring structure formation capacity in the angiogenesis assay upon VEGF-stimulation. MSCs from CAD patients do not seem to be inferior to MSCs from young healthy donors thus indicating that autologous MSCs may be suitable for cell therapy in CAD patients.

Key Words::

• Endothelial differentiation
• ischemic heart disease
• stem cell
• stem cell therapy

Acknowledgements

This work was supported by the Lundbeck Foundation, Aase and Ejnar Danielsen Foundation, the Research Foundation at Rigshospitalet and FP7 Health-2007-2.4.2.-5, grant no. 222995. The research nurse Sandra Miran and the technicians Lisbeth Egelykke Stolpe and Steen Miang Mortensen are thanked for technical assistance.

Declaration of interest: The authors report no conflict of interest. The authors alone are responsible for the content and writing of the article.