Preparation and Characterization of Hepatitis B-antigen for Radioimmunoassay: Scandinavian Journal of Clinical and Laboratory Investigation: Vol 32, No 3

Views

CrossRef citations to date

Altmetric

Abstract

A rapid and inexpensive method for purification of hepatitis B-antigen (HB-Ag) is described. By a precipitation procedure with polyethylenglycol 4000 HB-Ag can be separated from most serum proteins. Following gel filtration on a Sepharose 6B column immunologically pure antigen is obtained. For radioimmunoassay iodinated small spherical HB-Ag particles were found suitable as tracer. These particles were isolated by gel filtration on a Sepharose 4B column.

Key Words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Preparation and Characterization of Hepatitis B-antigen for Radioimmunoassay

Information for

Open access

Opportunities

Help and information

Preparation and Characterization of Hepatitis B-antigen for Radioimmunoassay

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature