Abstract
A highly sensitive and specific two-site solid-phase enzyme immunoassay for the quantitation of human immunoglobulin M (IgM) in biological fluids is presented. Detection limit is 20 μg/1 (12 ng/tube). Routine working range is 30–1000 μg/1. Between-assay coefficient of variation is 10%. The assay allows quantitation of IgM in human cerebrospinal fluid.