4
Views
3
CrossRef citations to date
0
Altmetric
Original Article

An enzymatic method of determining 7α-hydroxylated bile acids in urine

, &
Pages 765-767 | Received 06 Apr 1983, Accepted 02 Jul 1984, Published online: 08 Jul 2009
 

Abstract

A recently developed enzymatic method of determining unsulphated 7α-hydroxylated bile acids in serum was evaluated for the analysis of bile acids in urine after hydrolysis of the sulphate esters. Results obtained using the enzymatic method were compared to those obtained by radioimmunoassay of 7u-hydroxylated bile acids in urine samples from patients. Results of the enzymatic method were also compared to results of gas-liquid chromatography of individual bile acids after group separation of the bile acids on Sephadex-DEAP-LH-20. There was significant correlation between the enzymatic method and radioimmunoassay for bile acid determination in urine. The analysis of different conjugates after group separation of bile acids revealed no unspecific reactions when compared to gas-liquid chromatography. The reference range for 7α-hydroxylated urinary bile acids was in agreement with earlier published results.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.