Abstract
The effects of insulin in vitro on the total number of [3H]ouabain binding sites were studied in normal and concanavalin A-stimulated human T-lymphocytes after incubation for 18 and 42 h with 0, 102 and 105 mIU/l of insulin. While no effect on [3H]ouabain binding could be demonstrated in non-stimulated cells, a significant decrease could be produced in concanavalin A-stimulated cells. Mean±SD for the total number of [3H]ouabain binding sites per cell after 18 h incubation time with concanavalin A in the absence of insulin was 46,099±6,620 as compared with 44,783±8,347 in the presence of 102 mIU/l of insulin (nonsignificant) and 42,406±7,066 in the presence of 105 mIU/1 (p=0.031). The corresponding 42-h values were 47,075±9,412, 43,761 ±9,273 (p=0.033) and 43,824±9,312 (p=0.005).