Guidelines for routine measurement of blood hemoglobin oxygen affinity: International Federation of Clinical Chemistry (IFCC)

Abstract

Two methods for the routine determination of blood hemoglobin oxygen affinity are described. Both methods use whole blood and do not require special equipment, tonometry or special gas mixtures. The first method consists of a one-point determination of p₅₀, and requires only 200 μL to 400 μL of whole blood, therefore making it suitable for the pediatric population. The second method uses multiple points, thereby establishing both the shape and position of the hemoglobin oxygen equilibrium curve between 10 and 99 % oxygen saturation. Interpretation of p₅₀ is discussed in relation to evaluation of patients with hemoglobinopathies and as a parameter in estimating availability of oxygen to the tissues.

Key Words:

• Hemoglobin oxygen equilibrium curve
• Hill plot
• Bohr coefficient
• 2,3-diphosphoglycerate
• dyshemoglobins
• oxygen saturation
• oxyhemoglobin fraction
• blood gases
• hemoglobinopathy
• oxygen delivery.