Displaced chromatographic elution of gastrin-17 in normal human serum

Abstract

Gotze JP, Rehfeld JF. Displaced chromatographic elution of gastrin-17 in normal human serum. Scand J Clin Lab Invest 1994; 54: 673-6.

Sulfated gastrin-17 elutes by gel chromatography significantly earlier after incubation in human serum (K_D: 0.48) than when added to buffer (K_D: 0.62). Also, the elution of non-sulfated gastrin-17 changes after serum incubation although to a lesser extent (K_D: 0.68 vs. 0.74). In contrast neither the N-terminal 1-13 nor the sulfated C-terminal hexapeptide fragment of gastrin-17 change elution positions after serum incubation. The earlier elution is concentration dependent. The results suggest that displaced elution of sulfated gastrin-17 may constitute part of the abundance of gastrin-34-like material in normal human serum. Hence, present concepts of gastrin' heterogeneity in serum seem to require re-evaluation.

Key Words:

• gastrin
• gel filtration
• hormone heterogeneity
• tyrosine O-sulfation