204
Views
6
CrossRef citations to date
0
Altmetric
Short Report

Nitric oxide metabolites, nitrative stress, and paraoxonase activity in hepatopulmonary syndrome

, , , , &
Pages 73-77 | Received 25 Apr 2015, Accepted 05 May 2015, Published online: 07 Jun 2015
 

Abstract

Aim. To investigate possible abnormalities of vasoactive compounds, nitrative stress, and antioxidant activity of paraoxonase (PONa) in human hepatopulmonary syndrome (HPS), we determined endothelin-1 (ET), nitric oxide (NOx) metabolites, PONa alongside crude plasma nitrotyrosine (NT) as surrogate marker of nitrative stress. Material and methods. Liver cirrhosis (LC) patients with HPS (n = 12) were matched by age, sex, and Child–Pugh score to LC patients without HPS (n = 15) and to healthy controls (CTR) (n = 15); plasma NO2 (nitrite) (vascular metabolite), NO3 (nitrate) (inflammatory metabolite), and PONa were determined by a colorimetric assay, ET, and NT by immunoassays. Results. HPS patients showed higher level of ET (p = 0.0002), NO2 (p = 0.002), NO3 (p = 0.0001), NT (p < 0.0001), and lower PONa (p = 0.0004) than CTR; post-hoc analysis revealed greater ET (p < 0.05) and NO3 (p < 0.005) in LC patients with HPS than in LC patients without HPS. NT correlated to Child–Pugh score within HPS (p = 0.04) and LC (p = 0.02). Conclusion. Our HPS patients are characterized by elevated plasma levels of ET and NOx metabolites and lower PONa. Reduced PONa alongside elevated NO3 and NT suggests that defective antioxidation may favor nitrative stress and both may be implicated in the pathogenesis of HPS.

Acknowledgment

Supported by Regione Campania, Napoli, Italy, and by Med 2000, San Giorgio a Cremano, Napoli, Italy.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 336.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.