Typing of Candida isolates from patients with invasive infection and concomitant colonization

Abstract

We investigated the relationship between colonizing and invasive isolates from patients with candidaemia. Molecular typing was performed using random amplification of polymorphic DNA (RAPD) and multilocus sequence typing (MLST). We found MLST to be sufficient for typing Candida isolates, and that surveillance cultures are helpful in predicting concomitant invasive isolates, but not necessarily the pathogen involved in subsequent episodes.

Acknowledgements

We thank Annette Kørner and Birgit Brandt for excellent technical assistance in the preparation and testing of the specimens.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.