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Abstract
We retrospectively investigated 76 patients with cystic fibrosis for the presence of Pneumocystis jirovecii, by performing real-time PCR and nested-PCR assays on 146 archival sputum specimens. P. jirovecii was detected in only 1 patient (1.3%) showing that in our region (Brest, France), the fungus is rarely involved in pulmonary colonization in patients with cystic fibrosis.
Acknowledgements
This study was supported in part by “Vaincre la Mucoviscidose”, contract No. RCO 1773 and the Agence Française de Sécurité Sanitaire de l'Environnement et du Travail (AFSSET), contract No. EST-2006/1/41. The collection of archival specimens was registered with the French Ministry of Research and the Agence Régionale de l'Hospitalisation, No. DC-2008–214. This study was presented in part at the 10th International Workshop of Protists, May 2008, Boston, MA, USA. The authors thank Doctor Jean Le Bihan for his contribution.
Declaration of interest: No authors have conflicts of interest to report.