Chemiluminescence of Polymorphonuclear Leukocytes and Whole Blood during Acute Bacterial Infection

Abstract

The purpose of this study was to follow the changes in oxidative metabolism of polymorpho-nuclear leukocytes (PMN) and whole blood, during and after an acute bacterial infection, in otherwise healthy individuals, with the hypothesis that the majority of the subnormal activities found at clinical investigation of PMN functions in this respect, as part of the investigation of individuals with increased susceptibility to bacterial infections, is explained by subclinical infections or consequences of recent infections. 10 patients were followed from the day of admission and up to 80 days after the acute illness. Luminol- but not lutigenin-enhanced chemiluminescence (CL) of PMN was increased during the febrile period and normalized in parallel with normalization in body temperature. Both luminol- and lucigenin-enhanced CL were enhanced in whole blood during the period of fever. Subnormal activities of luminol- or ludgenin-enhanced CL were only seen sporadically. We conclude that the oxidative metabolism of PMN, as measured by lucigenin-enhanced CL, is virtually unaffected during acute bacterial infections but occasionally reduced during the postfebrife period. The cause of the increased luminol-enhanced CL during the acute illness is suggested to be due to the increased mobilization of myeloperoxidase.