Use of proton nmr spectroscopy to measure propofol metabolites in the urine of the female Caucasian patient

Abstract

1. Six female Caucasian patients received i.v. doses of propofol (2·7 ±0·3 (SE) mg/kg) for induction of anaesthesia. Anaesthesia was maintained with nitrous oxide, oxygen and enflurane for periods up to 3 h. A total urine collection was made from each subject for 24h after administration of propofol; samples were concentrated and analysed for propofol metabolites by nmr spectroscopy.

2. By 24h, 50·9 ± 4·0% of the dose of propofol was recovered as metabolites. The proportions of propofol metabolites, 1-quinol glucuronide (1-QG), 4-quinol glucuronide (4-QG), propofol glucuronide (PG) and 4-quinol sulphate (4-QS) recovered in urine (0-24 h) of the patients were 12 ± 1, 8 ± 2, 76 ± 4 and 4 ± 1% respectively. However, one patient showed a metabolite profile in which PG comprised 93% and 1-QG 7% of the total metabolites.

3. Nmr spectroscopy has been shown to he a satisfactory method for the quantification of propofol metabolites in urine without the necessity for reference samples.