In Vitro Responsiveness of Human Testicular DNA Synthesis to Gonadotropins

A. Okuyama Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

N. Nonomura Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

E. Koh Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

N. Kondoh Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

M. Nakamura Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

M. Namiki Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

H. Kiyohara Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

Y. Nishimune Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

K. Matsumoto Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

T. Sonoda Department of Urology and Pathology, Osaka University Medical School, the Research Institute for Microbial Diseases, Osaka University, and the Department of Urology, Osaka-Central Hospital, Osaka, Japan

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Abstract

To evaluate the responsiveness of spermatogenesis in the normal human testis to human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG), the DNA synthesis of spermatogonia was investigated using ³H-thymidine uptake by cultured tissue fragments of human testis with or without treatment with hCG and/or hMG. Two groups treated with different concentrations of hCG showed no difference in ³H-thymidine incorporation into testicular tissue compared with untreated control. In two other groups treatment with combined hMG/hCG in different concentrations induced significant changes in ³H-thymidine incorporation compared with control (p < 0.01, p < 0.01). Combined hMG/hCG treatment was effective in inducing spermatogenesis by normal human testicular tissue.

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In Vitro Responsiveness of Human Testicular DNA Synthesis to Gonadotropins

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In Vitro Responsiveness of Human Testicular DNA Synthesis to Gonadotropins

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