Effectiveness of Hmg-Hcg Treatment for DNA/RNA Syntheses in Subfertile Human Testis In Vitro

A. Okuyama Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

N. Nonomura Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

M. Nakamura Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

M. Namiki Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

E. Koh Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

N. Kondoh Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

H. Kiyohara Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

Y. Nishimune Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

K. Matsumoto Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

T. Sonoda Department of Urology and Pathology, Osaka University Medical School, Research Institute for Micro-bial Diseases, Osaka University, and Department of Urology, Osaka-Central Hospital, Osaka, Japan

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Abstract

To evaluate the effect of hMG-hCG treatment on spermatogenic impairment, DNA/RNA syntheses were measured in 25 patients with idiopathic male infertility by estimating the values of ³H-thymidine and ¹⁴C-uridine incorporation into the testicular tissue cultured with or without the hormonal treatment. Without the hormonal treatment, the levels of ³H-thymidine incorporation were in inverse correlation with the progress of spermatogenic impairment, whereas disturbances in responsiveness to the hormonal treatment directly correlated with spermatogenic impairment. Although the ¹⁴C-uridine incorporation increased both with and without the hormonal treatment, the values showed different patterns from those of ³H-thymidine incorporation. It would appear that the DNA synthesis in testicular tissue cultured with or without hMG-hCG treatment might predict the responsiveness to hormonal treatment for patients with idiopathic male infertility.

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Effectiveness of Hmg-Hcg Treatment for DNA/RNA Syntheses in Subfertile Human Testis In Vitro

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Effectiveness of Hmg-Hcg Treatment for DNA/RNA Syntheses in Subfertile Human Testis In Vitro

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