Computer-Assisted Sperm Motion Analysis of Bovine Sperm Treated with Insulin-Like Growth Factor I and II: Implications as Motility Regulators and Chemokinetic Factors

B. R. Lackey Endocrine Physiology Laboratory and Animal and Veterinary Science Department, Clemson University, Clemson, South Carolina, USA

W. R. Boone Animal and Veterinary Science Department, Clemson University, Clemson, South Carolina, USA; Andrology Laboratory, Reproductive Endocrinology and Infertility, Greenville Memorial Hospital, Greenville, South Carolina, USA

S. L. L. Gray Endocrine Physiology Laboratory and Animal and Veterinary Science Department, Clemson University, Clemson, South Carolina, USA

D. M. Henricks Endocrine Physiology Laboratory and Animal and Veterinary Science Department, Clemson University, Clemson, South Carolina, USACorrespondence[email protected]

Abstract

The effects of insulin-like growth factors (IGFs) I and II on motility of bovine sperm were examined using a computer-assisted sperm motion analyzer (CASA). The following kinematic parameters were examined: percentage of rapidly moving cells, straight-line velocity, curvilinear velocity, average path velocity, amplitude of lateral head displacement, and beat cross frequency. Sperm were treated with IGF-I (100 ng/mL) or IGF-II (250 ng/mL) and compared to sperm in modified Tyrodes' medium only (control) at 90, 180, and 360 min using CASA. Insulin-like growth factor I and II increased the percentage of rapidly moving cells, straight-line velocity, curvilinear velocity, average path velocity, amplitude of lateral head displacement, and beat cross frequency compared to the control treatment. These results indicate that IGFs may be involved in initiation and maintenance of bovine sperm motility.

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Computer-Assisted Sperm Motion Analysis of Bovine Sperm Treated with Insulin-Like Growth Factor I and II: Implications as Motility Regulators and Chemokinetic Factors

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Computer-Assisted Sperm Motion Analysis of Bovine Sperm Treated with Insulin-Like Growth Factor I and II: Implications as Motility Regulators and Chemokinetic Factors

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