16
Views
7
CrossRef citations to date
0
Altmetric
Original Article

Intracellular Particle Dissolution in Macrophages Isolated from the Lung of the Fischer (F-344) Rat

, , , &
Pages 143-156 | Received 30 Mar 1993, Accepted 22 Oct 1993, Published online: 02 Jul 2009
 

Abstract

Alveolar macrophages were removed from male F-344 rats by bronchoalveolar lavage and maintained in vitro for 14 days. Over this period the macrophages remained viable as judged by propidium iodide exclusion, lysosomal uptake of acridine orange, and phagocytosis of 1.75-μm latex beads. After 7 and 14 days of culture the cells contained lipid droplets. The macrophages were shown to ingest and dissolve monodisperse 57Co3O4 particles, which were relatively insoluble in extracellular medium. The fraction of 57CO3O4 dissolved intracellularly was determined at intervals during the culture period. The mean dissolution rate (± SEM) was 0.36 ± 0.02% per day for the F-344 rat. This was lower than 1.4 ± 0.05% per day estimated by Kreyling in macrophages from the beagle dog. The significance of this difference is discussed.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.