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Journal of Microencapsulation
Micro and Nano Carriers
Volume 27, 2010 - Issue 2
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Research Article

Preparation and immunological effectiveness of a Swine influenza DNA vaccine encapsulated in PLGA microspheres

Kai Zhao Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, PR China; National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China
,
Guo-Xin Li Shanghai Veterinary Research Institute, CAAS, Shanghai, PR China
,
Yuan-Yuan Jin Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, PR China; National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China
,
Hai-Xia Wei Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, PR China; National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China
,
Qing-Shen Sun Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, PR China
,
Ting-Ting Huang Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, PR China; National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China
,
Yun-Feng Wang National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China
&
Guang-Zhi Tong National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China; Shanghai Veterinary Research Institute, CAAS, Shanghai, PR ChinaCorrespondence[email protected]
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Pages 178-186 | Received 20 May 2009, Accepted 21 May 2009, Published online: 02 Feb 2010
 
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Abstract

Optimal preparation conditions of DNA vaccine against swine influenza encapsulated in Poly (D,L)-lactic-co-glycolic acid (PLGA) microspheres were determined. The microspheres were prepared by an emulsion-evaporation method using PLGA as the biodegradable matrix forming polymer. Using the optimal preparation conditions, PLGA microspheres containing the DNA vaccine were produced with good morphology as evident from scanning electron micrographs, high encapsulation rate and high stability. The transfection test indicated that the vaccine could be expressed as an antigen in cells and maintained good bioactivity. Moreover, these results demonstrated that the PLGA microspheres containing DNA vaccine can be used to achieve prolonged release of plasmid DNA. These results have laid a foundation for further development before ultimate industrial application.

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