Abstract
In Escherichia coli K1060 grown at 37°C we observed that the uptake of both L-[3H]leucine and L-[35S]methionine was inhibited by exposure of the cells to 48°C. The calcium channel Mockers diltiazem and verapamil, and the anti-arrhythmic agent quinidine, inhibited the uptake of L-[3H]leucine at both 37°C and 48°C. Verapamil also inhibited the uptake of L-[35S]methionine at 37°C, but at 48°C protected against some of the heat-induced decrease in the uptake of this amino acid. The local anaesthetic procaine markedly inhibited the uptake of both labelled amino acids at temperatures between 37°C and 48°C. Amino acid uptake and cell killing were not correlated.
Key Words: