Tacrolimus-Loaded PLGA Implants: In Vivo Release and Ocular Toxicity

Abstract

Purpose: To evaluate the in vivo release and ocular toxicity of a tacrolimus-loaded PLGA intravitreal implant.

Methods: Tacrolimus-loaded PLGA implants were inserted into the vitreous cavity of rabbits’ eye. At different time points, the vitreous was retrieved and the concentration of tacrolimus released from the implants was determined. Clinical examination was performed to evaluate the implant tolerance.

Results: PLGA implants provided controlled and prolonged release of tacrolimus. Approximately 99.97% of the drug was released from the devices at 6 weeks. Ophthalmic examination revealed no evidence of toxic effects of implants.

Conclusions: Tolerance and feasibility of the tacrolimus-loaded PLGA implants, as sustained intraocular drug delivery systems, were demonstrated.

• In vivo release
• noninfectious uveitis
• ocular drug delivery system
• PLGA
• tacrolimus

Acknowledgements

The authors wish to thank CNPq/MCT and Fapemig (Brazil) for financial support. The authors thank Dr Priscila Alves for English editing of the manuscript.