Comparative study of actin filament patterns in lens epithelial cells. Are these determined by the mechanisms of lens accommodation?

Abstract

Actin filament patterns in lens epithelia from animals of various taxonomic groups were studied using rhodamine phalloidin fluorescence microscopy of epithelial whole mounts and transmission electron microscopy of tangential sections. The results were compared with the accommodative mechanism operating in each case as reported in the literature.

Lenses that accommodate by deformation of the anterior surface, in squirrel, chipmunk, rabbit, monkey and human, showed polygonal arrays (PAS) at the apical end of the epithelial cells. Lenses that translate as a whole, in shark, bony fish and frog, showed stress fibers (SFs) at the basal or apical end of the cells. No specialized actin pattern was seen in turtle and bird, which have lenses that are squeezed into an anterior lenticonus; cat, where the lens is translated forward; or rat, cow and most mice, which have no defined accommodation. In exception, certain strains of laboratory mice did show sequestered actin bundles (SABs) and/or PAs. Based on our findings, we conclude that PAs, which resemble geodesic domes, do not take an active part in near-point accommodation; but like SFs, may serve to resist overextension by internal pressure of the fiber mass or by zonular tension.