Tear lipocalins bind a broad array of lipid ligands

Ben J. Glasgow Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, 90024, USA; Department of Pathology, UCLA School of Medicine, Los Angeles, CA, 90024, USA

Adil R. Abduragimov Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, 90024, USA

Zohreh Toossi Farahbakhsh Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, 90024, USA

Kym F. Faull Department of Chemistry & Biochemistry, UCLA School of Medicine, Los Angeles, CA, 90024, USA; Department of Psychiatry & Biobehavior Sciences, Neuropsychiatric Institute, and the Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, CA, 90024, USA

Wayne L. Hubbell Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, 90024, USA; Department of Chemistry & Biochemistry, UCLA School of Medicine, Los Angeles, CA, 90024, USA

Abstract

To identify the native ligands of tear lipocalins, tear proteins were separated by size exclusion chromatography and the lipid content in the major protein fractions identified. Lipids extracted from native tears and purified tear lipocalins co-migrated with fatty acids, fatty alcohols, phospholipids, glycolipids, and cholesterol on thin layer chromatograms. Abundant stearic and palmitic acids as well as cholesterol, and lesser amounts of lauric acid were specifically identified in extracts of purified lipocalins by gas chromatography-mass spectroscopy.

A preliminary study of the ligand-protein interaction was carried out using nitroxide spin-labeled lipids.

Key Words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Tear lipocalins bind a broad array of lipid ligands

Information for

Open access

Opportunities

Help and information

Tear lipocalins bind a broad array of lipid ligands

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature