Proteoglycans of Articular Cartilage From Bovine Lower Femoral Epiphysis: Extraction and Characterisation of Proteoglycans From Two Sites Within the Same Joint

Abstract

Bovine articular cartilages from two sites within the lower femoral epiphysis were extracted with GuHCl and purified by CsCl density gradient centrifugation in two steps as described by Hascall and Sajdera^1,2. Ninety per cent of the hexosamine containing material was extracted with 4 M GuHCl. The gel chromatographic properties of PGC and of PGS showed no or minor differences between the two sites. The amino acid, neutral sugar, uronic acid and hexosamine compositions were the same for the cartilage from the two sites.