Detection of the α-Thalassemia-2 (3.7 KB) Deletion in DNA Extracted from 20-Year-Old Blood Smears

J. E. Okeagu Department of Natural Sciences, Fayetteville State University, Fayetteville, NC, 28301, USA; Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA, 30912-2100, USA

N. S. Smetanina Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA, 30912-2100, USA

T. H. J. Huisman Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA, 30912-2100, USA

Abstract

Using a polymerase chain reaction procedure we have identified the 3.7 kb α-thalassemia-2 deletion in the DNA that was isolated from slides of blood smears stained with Wright's stain some 20 years ago. Details about the procedure are presented. The success of this approach depends entirely on the amount of DNA that could be isolated; thick smears always gave good data provided they were not covered with immersion oil. The success rate in this study was 45%.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Detection of the α-Thalassemia-2 (3.7 KB) Deletion in DNA Extracted from 20-Year-Old Blood Smears

Information for

Open access

Opportunities

Help and information

Detection of the α-Thalassemia-2 (3.7 KB) Deletion in DNA Extracted from 20-Year-Old Blood Smears

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature