In vitro skin permeation of hinokitiol loaded in vesicles composed of behenyltrimethylammonium chloride and stearic acid

Abstract

Introduction: Behenyltrimethylammonium chloride (BTAC) and stearic acid (SA) could be associated with each other through salt bridges, and the associated BTAC/SA could build bilayer vesicles with the aid of hinokitiol (HKL). Method: The vesicles were prepared by a precipitation method and used to enhance the skin permeation of HKL. Results: In case the molar ratio of BTAC/SA/HKL was 1/1/0, no vesicle was observed on transmission electron microscope photos. When the molar ratio of BTAC/SA/HKL was 1/1/0.4, vesicle was observed together with some agglomerates. When the content of HKL increased to the ratios of 1/1/0.8 and 1/1/1.2, vesicles were exclusively observed. In vitro fluxes for 18 hours through hairless mouse skin of HKL dissolved in alcoholic solutions were less than 1 mg/cm2/h. Whereas the fluxes of HKL encapsulated in the vesicles were about three times higher than that of HKL in the alcoholic solutions. Conclusion: The vesicles could be used for the hair growth promotion.

Keywords:

• Behenyltrimethylammonium chloride
• hinokitiol
• skin permeation
• stearic acid
• vesicles

Acknowledgments

This work was supported by a grant (20070401034013) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.