Development of Pasteurella multocida-loaded microparticles for hemorrhagic septicemia vaccine

Abstract

In this study, Pasteurella multocida-loaded alginate microparticles (MPs) for subcutaneous vaccination was developed by emulsification-cross-linking technique. Formulation parameter was varied as a ratio of polymer and bacterin. Optical microscopy revealed spherical particles with uniformly distribution. A mean particle size of approximately 6 µm has been successfully constructed using simple mixer and ultrasonic probe. The zeta potential of the MPs showed negatively charge of approximately −23 mV determined by Zeta Pals® analyzer. The entrapment efficiency and the in vitro bacterin released profile could be controlled by varying the amount of alginate. The high entrapment efficiency up to 69% was achieved with low concentration of alginate. The MPs possessed a slow bacterin release profile, up to 30 days. In vivo safety and potency tests were proved that the alginate MPs were safe and induced protective immunity in mice. In addition, after storage for 6 months at either 4 °C or room temperature, the protective immunity in mice was maintained.

• Alginate
• hemorrhagic septicemia
• microparticles
• Pasteurella multocida
• vaccine

Acknowledgements

We acknowledge Faculty of Pharmaceutical Sciences, Naresuan University for providing necessary facilities.

Declaration of interest

Financial support from the Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand and the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education, Thailand are gratefully acknowledge. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.