Optimization of a Reconstitutable Suspension of Rifampicin Using 2⁴ Factorial Design

Abstract

In this study 2⁴ factorial design associated with surface response methodology was used to develop and optimize a reconstitutable suspension of rifampicin. The study illustrated the effect of the percentage of each of sucrose, avicel RC-591, hydrophilic aerosil, and aerosol-OT on the flowability and the bulk density of the dry mixture as well as the viscosity, the sedimentation volume, and the redispersibility of the suspension. An empirical equation developed for each of the above responses was used with the aid of computer software to plot a contour map of the most significant effects and interactions. Five replicates at the center of the design were used to independently calculate the experimental error and to detect any curvature in the response surface. Three formulas which are not included in the design were prepared to check the validity of the model equation.