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ORIGINAL ARTICLECellular and Molecular Biology

Upregulation of SEPT7 Gene Inhibits Invasion of Human Glioma Cells

, , , , , , , & show all
Pages 248-258 | Published online: 16 Nov 2009
 

ABSTRACT

Objectives: To explore the role of SEPT7 in glioma cell invasion. Methods: SEPT7 was transfected into human glioma cell lines U251 and TJ899, the invasive abilities were evaluated by transwell assay, scratch assay, and 3-D/2-D Matrigel growth. The expression of MMP2/9, MT1-MMP, integrin αvβ3, and TIMP1/2 was detected by immunohistochemistry, immunofluorescence, and Western blot analyses. Distribution of α-tubulin was examined by laser scanning confocal analysis. Result: After SEPT7 trasfection, cell invasion was inhibited, expression of MMP2/9, MT1-MMP, and integrin αvβ3 was decreased, while TIMP1/2 was increased, and α-tubulin was redistributed. Conclusion: These results suggest that SEPT7 plays an important role in the glioma cell invasion.

Keywords: :

ACKNOWLEDGMENTS

This work is supported by National Natural Science Foundation of China, grant numbers 30500523 and 30872985; Tianjin Science and Technology Committee, grant number 06YFSZSF01100; Tianjin Education Committee, grant number 20070234; and Program for New Century Excellent Talents in University (NCET-07-0615). We thank Eline Menu for the assistance with language corrections.

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