Angiogenesis Inhibition Using an Oncolytic Herpes Simplex Virus Expressing Endostatin in a Murine Lung Cancer Model

Abstract

Herpes-mediated viral oncolysis alone is not sufficient to completely eradicate tumors. In this study we used a replication conditional, endostatin-expressing herpes simplex virus-1 mutant (HSV-Endo) in a murine lung cancer model. We hypothesized that the anti-angiogenic action of endostatin would improve upon the oncolytic effect of HSV-1. HSV-Endo was evaluated in a pulmonary metastases and orthotopic flank model, where there was significantly less tumor burden and reduced microvessel density compared to a control virus. Endostatin expression appears to improve the anti-tumor effect of HSV-1 in a lung cancer model.

Keywords: :

• Herpes simplex virus
• Viral oncolysis
• Endostatin
• Angiogenesis
• Lung cancer

ACKNOWLEDGMENT

This work was supported by departmental funds from the Department of Surgery, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, MA.