“Axitinib Affects Cell Viability and Migration of a Primary Foetal Lung Adenocarcinoma Culture”

Abstract

Fetal lung adenocarcinoma (FLAC) is a rare variant of lung adenocarcinoma. Studies regarding FLAC have been based only on histopathological observations, thus representative in vitro models of FLAC cultures are unavailable. We have established and characterized a human primary FLAC cell culture, exploring its biology, chemosensitivity, and migration. FLAC cells and specimen showed significant upregulation of VEGF₁₆₅ and HIF-1α mRNA levels. This observation was confirmed by in vitro chemosensitivity and migration assay, showing that only Axitinib was comparable to Cisplatin treatment. We provide a suitable in vitro model to further investigate the nature of this rare type of cancer.

Keywords: :

• Fetal lung adenocarcinoma
• Lung adenocarcinoma
• Vascular endothelial growth factor
• Axitinib
• Antineoplastic drugs
• Cancer primary cell cultures

ACKNOWLEDGMENTS

The authors thank Dr. Luigi Ruco, Dr. Rosanna Boccia, and Dr. Elisabetta Grigioni for their support. They also acknowledge Fondazione Roma.