Overexpression of miR-145–5p Inhibits Proliferation of Prostate Cancer Cells and Reduces SOX2 Expression

ABSTRACT

We aimed to perform functional analysis of miR-145–5p in prostate cancer (PCa) cells and to identify targets of miR-145–5p for understanding its role in PCa pathogenesis. PC3, DU145, LNCaP PCa, and PNT1a nontumorigenic prostate cell lines were utilized for functional analysis of miR-145–5p. Its overexpression caused inhibition of proliferation through apoptosis and reduced migration in PCa cells. SOX2 expression was significantly decreased in both mRNA and protein level in miR-145–5p-overexpressed PCa cells. We proposed that miR-145–5p, being an important regulator of SOX2, carries a crucial role in PCa tumorigenesis.

Keywords: :

• hsa-miR-145–5p
• SOX2
• Prostate cancer
• Stem cell marker
• Cancer stem cell