Knocking down Dp71 expression in A549 cells reduces its malignancy in vivo and in vitro

ABSTRACT

Dp71 is one of the most ubiquitously expressed isoforms of dystrophin, the pathological genes of DMD. In order to find whether the alteration of Dp71 can affect the phenotypes of cell other than PC12, an A549 cell line with stably transfected Dp71 siRNA plasmids was set up and named A549-Dp71AS cell. It is demonstrated for the first time that the A549-Dp71AS cell line displayed decreased invasion capabilities, reduced migration ability, decreased proliferation rate, and lessened clonogenic formation. Cisplatin-induced apoptosis was also increased in A549-Dp71AS cell line via enhancing the Caspase 3, Caspase 8, and Caspase 9 activities. Knocking down Dp71 expression can significantly inhibit the A549 xenograft tumor growth in nude mice. The A549-Dp71AS cells and xenograft tumor tissues displayed reduced lamin B1, Bcl-2, and MMP2 protein expression, which accounts for the reduced malignancy of A549-Dp71AS cells in vivo and in vitro.

Keywords:

• Dp71
• Knockdown
• A549
• Malignancy

Acknowledgments

This study is supported by the National Natural Science Fund of China (Grant No. 30800550), Hunan Natural Science Fund of China (Grant No.10JJ4016), and the Open-End Fund for the Valuable and Precision Instruments of Central South University.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.