Phase and period responses to short light pulses in a wild diurnal rodent, Funambulus pennanti

Abstract

Photic phase response curves (PRCs) have been extensively studied in many laboratory-bred diurnal and nocturnal rodents. However, comparatively fewer studies have addressed the effects of photic cues on wild diurnal mammals. Hence, we studied the effects of short durations of light pulses on the circadian systems of the diurnal Indian Palm squirrel, Funambulus pennanti. Adult males entrained to a light–dark cycle (12 h–12 h) were transferred to constant darkness (DD). Free-running animals were exposed to brief light pulses (250 lux) of 15 min, 3 circadian hours (CT) apart (CT 0, 3, 6, 9, 12, 15, 18 and 21). Phase shifts evoked at different phases were plotted against CT and a PRC was constructed. F. pennanti exhibited phase-dependent phase shifts at all the CTs studied, and the PRC obtained was of type 1 at the intensity of light used. Phase advances were evoked during the early subjective day and late subjective night, while phase delays occurred during the late subjective day and early subjective night, with maximum phase delay at CT 15 (−2.04 ± 0.23 h), and maximum phase advance at CT 21 (1.88 ± 0.31 h). No dead zone was seen at this resolution. The free-running period of the rhythm was concurrently lengthened (deceleration) during the late subjective day and early subjective night, while period shortening (acceleration) occurred during the late subjective night. The maximum deceleration was noticed at CT 15 (−0.40 ± 0.09 h) and the maximum acceleration at CT 21 (0.39 ± 0.07 h). A significant positive correlation exists between the phase shifts and the period changes (r = 0.684, p = 0.001). The shapes of both the PRC and period response curve (τRC) qualitatively resemble each other. This suggests that the palm squirrel’s circadian system is entrained both by phase and period responses to light. Thus, F. pennanti exhibits robust clock-resetting in response to light pulses.

• Circadian rhythm
• entrainment
• free-running
• phase shift
• PRC
• τRC

Acknowledgments

Financial support by CSIR, New Delhi to MS (Project No: 37 (1283)/07/EMR-II), ICMR to DJ as SRF is gratefully acknowledged. UGC-NRCBS, SBS, MKU Madurai is acknowledged for technical support. We thank Mr. Punit Kumar for animal handling and their maintenance during the experiment.