Publication Cover
Chronobiology International
The Journal of Biological and Medical Rhythm Research
Volume 7, 1990 - Issue 3
4
Views
4
CrossRef citations to date
0
Altmetric
Original Article

The Use of Robots and Computers in the Organisation of Studies on the orcadian Variation of β2-Adrenoceptor Sites in Peripheral Mononuclear Leucocytes

, , , &
Pages 235-238 | Published online: 07 Jul 2009
 

Abstract

We have developed a partially automated method for the performance of equilibrium radioligand binding studies which is applied by our group in investigations on circadian variations and stimulation studies on β2 adrenoceptor sites in human peripheral mononuclear leucocytes (pMNL). Using a Tecan Robotic Sample Processor, binding assays with 12 concentrations of ' iodocyanopindolol (1–150 pmol/1, total binding in triplicates, unspecific binding in the presence of 10-5 mol/l timolol in duplicates) are prepared automatically with all titer tubes per experiment arranged in a microtiterplate-sized rack. After incubation in a waterbath for 2hr at 37o C, the whole rack is centrifuged at 500% and transferred back to the lab robot. Bound radioactivity is separated from the unbound ligand by removing the supernatant by the machine. The radioactive counts are evaluated using personal computers. The lab robot enhances reproducibility of experimental results and frees lab workers from time-consuming pipetting jobs. Radioactive exposure is minimized to the time preparing the radioligand working solution and transferring the sample tubes from the robot to the waterbath, to the centrifuge and back to the robot. The variability of our software allows easy adaptation to other binding studies with intact cells.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.