A Simple, Sensitive, Rapid and Specific Detection Method for Bisphenol A based on Fluorescence Polarization Immunoassay

Abstract

A rapid, sensitive, specific and simple method based on Fluorescence Polarization Immunoassay (FPIA) was developed for the quantitative detection of Bisphenol A in water. 4, 4-Bis (4-hydroxyphenyl) valeric acid (BHPVA) was selected as the hapten to produce the immunogen. Fluorescein-labeled Bisphenol A derivatives (tracers) with different structures were synthesized and purified by thin layer chromatography. Based on the polyclonal antibody and tracers, an optimized FPIA method was developed with a detection limit (10% inhibition) of 2 μg L⁻¹ and a linear working range of 20 to 800 μg L⁻¹. FPIA was suitable for screening a large number of samples. The recoveries of fortified Tai lake water samples ranged from 91.85 to 102.78%, and tap water samples ranged from 90.36 to 96.01%. The coefficients of variation were all less than 20%. This FPIA method, which did not require a complicated cleanup process, proved to be very useful for the screening of Bisphenol A in environmental water samples.

• Bisphenol A
• Fluorescence polarization immunoassay
• Tracer synthesis
• Water detection

ACKNOWLEDGMENT

This work is financially supported by the National Natural Science Foundation of China (21071066, 20835006, 91027038), the 11th Five Years Key Programs for Science and Technology Development of China (2010AA06Z302, 2008BAK41B03, 2009BAK61B04, 2008ZX08012-001, 2010GB2C100167), and grants from Natural Science Foundation of Jiangsu Province, MOF and MOE (BK2010001, BK2010141, 2010DFB3047, JUSRP11019, 201110060, 201110016, 201110061, 201010078, 201010216, 200910013, 200910083, 200910011, 200910277).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.