Six Alkaloids Inhibit Secretion of IL-1α, TXB₂, ET-1 and E-selectin in LPS-induced Endothelial Cells

Abstract

The aim of the research was to investigate the antiendotoxin effects of Sinomenine, Fangchinoline, Stachydrine, Chuanxionggzine, Oxymartrine and Evodiamine. Endothelial cells were challenged with 1 μg/mL LPS for 3 h then treated respectively with six alkaloids at three concentrations (1, 5 and 10 μg/mL). The cells were incubated at 37°C in a cell incubator for 21 h. The supernatants were collected and analyzed the levels of interleukin-1α (IL-1α), thromboxane B₂ (TXB₂), endothelin-1 (ET-1) and E-selectin by ELISA kits. The results revealed that Sinomenine, Oxymartrine and Evodiamine inhibited the production of IL-1α; Stachydrine, Chuanxionggzine and Evodiamine inhibited the secretion of TXB₂; Sinomenine and Oxymartrine down-regulated ET-1 expression; Fangchinoline and Evodiamine decreased the level of E-selectin. All these changes were significant. Taken together, the data suggested that six alkaloids may effectively reduce inflammatory response via these cytokines.

• IL-1α
• TXB₂
• ET-1
• E-selectin
• LPS
• Alkaloid

ACKNOWLEDGMENTS

This project was supported by Jiangsu Agriculture Technology Independent Innovation Foundation (Grant No. cx425). We greatly appreciate the help we received from all of our colleagues and collaborators in these experiments.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.