Significant Impact of IL-6 -174G/C but Inverse Relation with -634 C/G Polymorphism in Patients with Non-Small Cell Lung Cancer in Kashmiri Population

Abstract

To study the possible role of proinflammatory interleukin 6 -174 G>C (rs 1800795) and -634 C>G (rs 1800796) polymorphism in the pathogenesis of non-small cell lung cancer (NSCLC). A total of 190 NSCLC patients and 200 healthy controls were evaluated for polymorphic analysis of -174 G/C and -634 C/G by PCR-RFLP followed by DNA sequencing. A significant association was observed in the genotypic and allelic distribution of IL-6 -174 G/C in the NSCLC group as compared to control group [OR = 2.7 (1.77–4.11), p < 0.0001]. Smokers with the -174C allele were found to be significantly associated with NSCLC (p = 0.01), while 634C/G SNP showed an inverse relation [OR-0.4, p < 0.0001]. The present investigation revealed a significant association of the IL6 -174 G/C gene promoter polymorphism with NSCLC, and thus, the IL-6 -174G/C genotype can be considered as one of the biological markers in the etiology of NSCLC.

• Inflammation
• non-small cell lung cancer
• polymorphism
• restriction fragment length polymorphism (RFLP)

Acknowledgements

The authors gratefully acknowledge the financial support provided by the Sher-i-Kashmir Institute of Medical Sciences, Srinagar, for this work. Our thanks are also due to the technical staff of Medical Oncology and Cardiovascular and Thoracic Surgery, SKIMS, Srinagar, who helped us in procuring the NSCLC samples.

Declaration of interest

The authors have no conflict of interest and nothing to disclose.