Sulfated Radix Cyathulae officinalis Polysaccharides Act as Adjuvant via Promoting the Dendritic Cell Maturation and Suppressing Treg Frequency

Abstract

This study was conducted to evaluate the adjuvant potential of sulfated Radix Cyathulae officinalis Kuan polysaccharides (sRCPS) and their effects on specific cellular and humoral immune responses to hepatitis B subunit vaccine in mice. Our data demonstrated that sRCPS significantly promoted the rHBsAg-specific IgG, IgG1, IgG2a, and IgG2b antibody titers, the activities of natural killer cells (NK) and cytotoxic T lymphocytes (CTL), T cells proliferation, and phagocytic capacity of peritoneal macrophages. Furthermore, sRCPS increased the levels of IL-4, IL-2, and IFN-γ in CD4⁺T cells and the level of IFN-γ in CD8⁺T cells. In addition, sRCPS enhanced the expression of CD40⁺, CD80⁺, CD86⁺, MHC I and MHC II in dendritic cells (DCs) and upregulated the mRNA levels of MHC I, MHC II. sRCPS downregulated the frequency of CD4⁺CD25⁺Foxp3⁺ Treg cells. sRCPS increased both cellular and humoral immune responses by upregulating DC maturation, and suppressing the frequency of Treg cells.

• Adjuvant
• dendritic cell
• radix cyathula polysaccharide
• regulatory T cell
• sulfated modification

Acknowledgements

This study was supported by the national Natural Science Foundation of China (Project Nos. 31402238 and 31172357) and in part by the Fundamental Research Funds for the Central Universities (Project No. XDJK2013C136 and SWU113091).

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.