Abstract
Zone electrophoresis of anti–human sperm antibodies elicited in chickens and isolated from the yolks of eggs laid by the birds were subjected to zone electrophoresis in sucrose con= centration gradients. Because of its very low migration rate in the electric field it was possible to isolate the specific IgY antibody component in a virtually pure state. In zone electrophoresis experiments on human “auto” anti–sperm antibodies the presence of both IgA and IgG types was shown in human Ig. The IgA agglutinating antibody was 1/6 that of the IgG component. The mobility in the electric field of the IgG-type antibody was very low, similar to the IgY-type human sperm anti body.