Activity of Serum Enzymes in Puromycin Aminonucleoside-Induced Nephrotic Syndrome

Abstract

Total serum protein, serum albumin, total urine protein excretion, and the serum activity of several enzymes—aldolase (ALS), cholinesterase (CHS), leucine aminopeptidase (LAP), isocitrate dehydrogenase (ICD), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), å-hydroxy-butyrate dehydrogenase (HBD), creatine kinase (CK), alkaline phosphatase (ALP), and y-glutamyl transferase (GGT)—were estimated in rats with nephrotic syndrome (NS) at 2,4, 6, 8, 10, 12, 16, 20, and 30 days after a single injection of puromycin aminonucleoside (PAN). It was found that: (a) total serum protein and serum albumin diminished on day 4 and returned to control values on days 20 and 30, respectively; (b) total urine protein excretion rose on day 4, reached a peak value on day 8, and then fell substantially but still remained higher than control values on day 30; (c) ALS and CHS activities increased; (d) LAP, ICD, and AST activities showed a biphasic pattern, first increasing and then decreasing; (e) ALT, LDH, HBD, CK, and ALP activities decreased; and (f) GGT activity remained unchanged. The differences in the profiles of the enzyme activities suggest their independent regulation in experimental NS induced by PAN.