Tigecycline attenuates staphylococcal superantigen-induced T-cell proliferation and production of cytokines and chemokines

Abstract

The purpose of this study is to examine the in vitro modulatory effect of tigecycline on staphylococcal superantigen-induced T-cell activation and cytokines and chemokines production by human peripheral blood mononuclear cells (PBMC). Isolated human PBMC from ten healthy volunteers were stimulated by staphylococcal enterotoxin B (SEB) and Staphylococcal toxic shock syndrome toxin-1 (TSST-1) superantigens with varying concentrations of tigecycline. Cytokines IL-1β, IL-6, TNF-α, and chemokines MIP-1α and MIP-1β concentrations were measured along with T cell proliferation. Results demonstrated that tigecycline alters cytokine production and reduces T-cell proliferation in vitro suggesting an immunomodulatory activity independent of its antimicrobial effect.

Keywords::

• Glycyclines
• antinflammatory
• cytokines
• cell proliferation

Acknowledgments

Funding for the study was supported by a grant no. 3074A1 - 4422 from Wyeth Pharmaceuticals, Wayne, New Jersey, USA.

Declaration of interest: The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.