Interleukin-1β and surface marker expression changes induced by tetrachloroplatinate in human monocyte-derived dendritic cells

Abstract

Background and aim: Platinum salts, known occupational respiratory sensitizers, may also induce contact hypersensitivity in animal models. The aim of this study was to determine the effect of potassium tetrachloroplatinate (TCPP) on in vitro cultured human monocyte-derived dendritic cells (MoDCs) and to compare it with a response to nickel sulfate (NiSO4). The expression of CD54, CD86, and HLA-DR surface antigens as well as IL-1β transcription was investigated.

Conclusions: MoDCs populations generated from three healthy volunteers displayed the phenotype responsive to NiSO4 and non-responsive to sodium dodecyl sulfate (SDS). Clear up-regulation of IL-1β mRNA as well as CD54, CD86 and HLA-DR expression in response to NiSO4 (250 μM) was detected after 48 hrs of exposure. No up-regulation of evaluated surface antigens was observed following the treatment with SDS (150 μM). TCPP (150 μM) similarly to NiSO4 induced up-regulation of IL-1β mRNA, CD54, CD86, but not HLA-DR expression. The response to TCPP showed less inter-individual variability, however it was weaker in comparison to the response to NiSO4. The results suggest that platinum salts can induce in MoDCs phenotypical changes characteristic for skin sensitizers.

Keywords::

• Interleukin-1β
• surface markers
• tetrachloroplatinate
• sensitizers
• dendritic cells

Acknowledgments

The authors gratefully acknowledge Dr Jarosław Dastych, Associate Professor in the Laboratory of Cellular Immunology at the Institute for Medical Biology, Polish Academy of Sciences, and Dr Waldemar Wagner, Assistant Professor in the laboratory for their assistance in FACS analysis.

Declaration of interest: This work was supported by the Nafer Institute of Occupational Medicine, grant Nr. IMP1.12/2007.