Combination of Adoptive Cell Transfer and Antibody Injection Can Eradicate Established Tumors in Mice–An in vivo study using anti-OX40mAb, anti-CD25mAb and anti-CTLA4mAb–

Abstract

Boosting an effective immune response against established tumors remains a difficult challenge. This study shows the combination of 1) adoptive cell transfer using CD25 depleted splenocytes co-cultured with irradiated tumor cells, and 2) antibody injection therapy using CTLA4 blockade, the elimination of Treg and OX40, which together could eradicate an established MethA tumor in over 50% of the BALB/c mice. Each element of the protocol was shown to be necessary, as elimination of any factor except anti-CD25 antibody injection failed to eradicate the tumor.

Keywords::

• CD25
• CTLA4
• OX40
• Tumor Immunology
• Tumor rejection

Acknowledgments

The authors would like to thank Kathryn Wood for her help in writing this manuscript.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.