Leaf extracts from Phlomis crinita Cav. subs. mauritanica Munby affect immune cell functions in vitro

Abstract

Aqueous and methanolic leaf extracts from Phlomis crinita subs. mauritanica Munby were investigated for their potential immunomodulatory activity on mouse lymphocytes and macrophages in vitro. The phagocytic activity of macrophages and the proliferation of lymphocytes in the absence and presence of mitogens (lipopolysaccharide, LPS or lectin) were assayed. Depending on the concentrations, the extracts affect macrophage functions by modulating their lysosomal enzyme activity and nitric oxide release. For lymphocyte proliferation assay, both extracts enhance significantly cell proliferation either with or without mitogen stimulation. These results suggest that leaf extracts from P. crinita subs. mauritanica Munby contain potent components such as flavonoids that potentially may be useful for modulating immune cell functions in physiological and pathological conditions.

Keywords::

• Phlomis crinita Cav. subs. mauritanica
• nitric oxide
• cell proliferation
• lysosomal enzyme activity
• immunomodulation